Exam 2 Study Guide 2008

Ch 8: DNA: The Molecule of Heredity

·         Name the 3 parts of a nucleotide;

·         Show how nucleotides bonded to form macromolecules (where covalent, hydrogen);

·         Understand the structure of DNA: the Watson-Crick model;

·         Say why people once thought that proteins were the genetic material;

·         Use the base-pairing rules to predict the structure of a complementary strand of DNA;

·         Explain why DNA replication is called “semi-conservative replication”;

·         List the events that take place in DNA replication, with the associated enzymes;

·         Explain the importance of proofreading in DNA replication;

Ch 9: DNA to Proteins

·         Say what a gene is;

·         Discuss the relationship between genes and proteins, including transcript modification in your discussion;

·         Differentiate between DNA and RNA in terms of strand number, kinds of bases, sugar present, location in the cell;

·         Understand the differences between transcription and translation;

·         List the types of RNA, their location in the cell and their function;

·         Say what a codon and an anticodon are, and be able to predict them from a DNA sequence;

·         List the events that take place in transcription, with the associated enzyme;

·         List the events that take place in translation, and understand the role of each type of RNA in those events;

·         Predict the amino sequence of a protein from mRNA if given a table of correspondences between codons and amino acids;

·         Understand what a mutation is, and the mechanisms by which they are produced;

·         Explain how mutations differ in their effects on protein structure and function;

Ch 10: Cell Reproduction

·         Tell what is the difference between sexual and asexual reproduction (What is the end result? What type of cell division is employed?);

·         Describe the process of binary fission;

·         Distinguish between a chromatid and a chromosome;

·         Name the stages on a cell cycle diagram and recognize what is happening at each stage;

·         List the stages of mitosis and describe what happens to the DNA in each stage;

·         Recognize the stages of mitosis when given a picture of a cell undergoing mitosis;

·         Describe the daughter cells that result from mitosis compare with the parent cells;

·         Explain how cytokinesis is different in plant and animal cells;

·         Tell what a homologous chromosome is, and where they are found (in gametes, for example?);

·         Differentiate between haploid and diploid cells;

·         Explain what type of cell division is used to form gametes;

·         Tell what crossing over is and when it occurs;

·         Explain what would happen if gametes did not go through meiosis and become haploid;

·         Explain what the main point of meiosis is;

·         Explain what independent assortment is;

·         Tell what three things contribute to the production of offspring with new combinations of alleles (and thus variation);

·         Distinguish between mitosis and meiosis (What is the end product of each? What is the purpose of each?);

Ch 11: Mendelian Genetics

·         Understand the terminology of genetics (genotype, phenotype, homozygous, heterozygous, alleles, locus (loci), F1, F2, monohybrid, dihybrid, etc.);

·         Distinguish between dominant and recessive traits;

·         Explain Mendel’s principles of segregation and independent assortment, and say why they occur;

·         Use a Punnett square to determine the ratios of genotypes and phenotypes for monohybrid crosses;

·         Use a Punnett square to determine the ratios of phenotypes for dihybrid crosses;

·         Recognize how incomplete dominance, polygenic inheritance, pleiotropy, and environmental effects may change simple Mendelian ratios;

·         Determine gametes from genotypes where genes are linked or non-linked;

·         Define linkage and sex (X) linkage;

·         Describe sex determination in humans;

·         Recognize the outcomes of changes in chromosome structure and number;

·         Solve simple genetic problems involving dominance, recessiveness, incomplete dominance, codominance, and sex linkage (hemophilia and color blindness);

Ch 12: Biotechnology

·         Explain the types of genetics experiments that nature has been performing for billions of years;

·         Recognize how PCR and gel electrophoresis are used in forensics;

·         Understand how transgenic organisms are created;

·         Understand what plasmids are and how they may be used to insert new genes into recombinant DNA molecules;

·         Explain, using examples, why you believe that human "tinkering" with genes in different organisms (GMO’s) is primarily a benefit, or a disaster about happen;

·        Discuss the human genome initiative and its ethical implications;

·     Discuss the unity of life’s genetic code, incorporating your 50% relatedness to a banana, how one organism can produce the products of another, and sequences that match from you to yeast; 

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