C. Estrella

Biology 4

Chapter 20 Learning Objectives DNA Technology

1. Explain how advances in recombinant DNA technology have helped scientists study the eukaryotic genome.
2. Describe the natural function of restriction enzymes.
3. Describe how restriction enzymes and gel electrophoresis are used to isolate DNA fragments.
4. Explain how the creation of sticky ends by restriction enzymes is useful in producing a recombinant DNA molecule.
5. Outline the procedures for producing plasmid and phage vectors.
6. Explain how vectors are used in recombinant DNA technology.
7. List and describe the two major sources of genes for cloning.
8. Describe the function of reverse transcriptase in retroviruses and explain how they are useful in recombinant DNA technology.
9. Describe how “genes of interest” can be identified with the use of a probe.
10. Explain the importance of DNA synthesis and sequencing to modern studies of eukaryotic genomes.
11. Describe how bacteria can be induced to produce eukaryotic gene products.
12. Explain how PCR can be applied to the Human Genome Project.
13. Describe how recombinant DNA technology can have medical applications such as diagnosis of medical diseases, development of gene therapy, vaccine production, and development of pharmaceutical products.
14. Describe how gene manipulation has practical applications for agriculture.